5TBN

Solution NMR structure of PHF20 PHD domain in complex with a histone H3K4me2 peptide

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE III		700

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	3D 1H-15N NOESY
11	1	2	3D 1H-13C NOESY aliphatic
12	1	2	3D 1H-13C NOESY aromatic
13	1	2	3D 13C,15N-filtered, 13C/15N-edited NOESY
14	1	3	2D 1H-13C HSQC
15	1	3	2D 1H-1H NOESY
16	1	3	2D 1H-1H COSY
2	1	1	3D 1H-15N NOESY
3	1	2	2D 1H-13C HSQC
4	1	2	3D HNCACB
5	1	2	3D CBCA(CO)NH
6	1	2	3D HNCO
7	1	2	3D NH(CA)CO
8	1	2	3D HBHA(CO)NH
9	1	2	3D CCH-TOCSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7	101325	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20