5TBH
Crystal structure of mouse CARM1 in complex with inhibitor LH1236
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.647, 98.529, 206.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.260 - 2.341 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ih3 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.024 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1980) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.260 | 2.400 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.275 | 1.836 |
Number of reflections | 64779 | |
<I/σ(I)> | 7.4 | 1.8 |
Completeness [%] | 99.6 | 90.9 |
Redundancy | 6.3 | 5.9 |
CC(1/2) | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM PEG 2000 MME 19% NaCl 100 mM |