5TBF
Crystal structure of SeMet derivatives of domain2 and domain 3 of RctB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-19 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.526, 105.526, 137.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.370 - 3.003 |
| R-factor | 0.2223 |
| Rwork | 0.220 |
| R-free | 0.27830 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.859 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev-2376_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 18054 |
| <I/σ(I)> | 19.68 |
| Completeness [%] | 99.1 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 1.5M Ammonium Sulfate |
