5TBB
Postcatalytic ternary complex of Human DNA Polymerase Beta with Gapped DNA substrate, incorporated (-)FTC and PPi.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.170, 79.330, 55.460 |
| Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
| Resolution | 44.520 - 2.390 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rq7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.535 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.520 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.094 | 0.474 |
| Number of reflections | 18179 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM ACETATE, 17% PEG3350, PH 8.0, |
