5TBA
Postcatalytic ternary complex of Human DNA Polymerase Beta with Gapped DNA substrate, incorporated (-)3TC and PPi.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2013-10-11 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.697, 79.325, 54.947 |
Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
Resolution | 25.180 - 2.490 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rq7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.464 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.180 | 2.590 |
High resolution limit [Å] | 2.490 | 2.500 |
Rmerge | 0.168 | 0.708 |
Number of reflections | 15629 | |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 5.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM ACETATE, 17% PEG3350 , PH 8.0 |