5T9Y
Crystal structure of the infectious salmon anemia virus (ISAV) hemagglutinin-esterase protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-28 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.419, 93.289, 96.722 |
Unit cell angles | 90.00, 122.01, 90.00 |
Refinement procedure
Resolution | 47.720 - 1.800 |
R-factor | 0.1373 |
Rwork | 0.137 |
R-free | 0.16770 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.720 | 47.720 | 1.830 |
High resolution limit [Å] | 1.800 | 9.860 | 1.800 |
Rmerge | 0.057 | 0.029 | 0.498 |
Number of reflections | 99190 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 99.9 | 98.7 | 100 |
Redundancy | 4.2 | 3.9 | 4.2 |
CC(1/2) | 0.999 | 0.998 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | [0.2 M] magnesium formate, 20% PEG 3350, w/ a [0.8 M] sodium phosphate, [0.8 M] potassium phosphate, [0.1 M] HEPES pH 7.5 solution as a 1:6 ratio drop additive |