5T9T
Protocadherin Gamma B2 extracellular cadherin domains 1-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.722, 118.067, 98.190 |
Unit cell angles | 90.00, 103.91, 90.00 |
Refinement procedure
Resolution | 39.751 - 3.500 |
R-factor | 0.2151 |
Rwork | 0.213 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5szr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.613 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.780 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.369 | 1.176 |
Number of reflections | 22663 | |
<I/σ(I)> | 4.8 | 1.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.957 | 0.561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 295 | 8.3% (w/v) PEG8000, 16.7% (v/v) ethylene glycol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1 M Morpheus Buffer System 2 (Hepes/MOPS buffer; Molecular Dimensions) |