5T9F

Prephenate Dehydrogenase N222D mutant from Soybean

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	46.445, 55.046, 68.387
Unit cell angles	107.82, 99.56, 102.58

Refinement procedure

Resolution	33.846 - 1.994
R-factor	0.1602
Rwork	0.158
R-free	0.20580
RMSD bond length	0.008
RMSD bond angle	0.912
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.850	2.048
High resolution limit [Å]	1.994	1.994
Number of reflections	36694
<I/σ(I)>	10.19
Completeness [%]	88.9	85.3
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2 mM of an oxometalates solution containing 0.005 M sodium chromate tetrahydrate, 0.005 M sodium molybdate dihydrate, 0.005M sodium tungstate dihydrate, and 0.005 M sodium orthovanadate, 0.1 M of MOPSO and Bis-Tris, pH 6.5, and 50% v/v of a precipitant mixture of 20% w/v PEG 8000 and 40% v/v 1,5-pentanediol