5T9C

Crystal structure of B. subtilis 168 GlpQ in complex with glycerol-3-phosphate (1 hour soak)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-09
Detector	RAYONIX MX-300
Wavelength(s)	1.03322
Spacegroup name	P 21 21 21
Unit cell lengths	47.101, 58.240, 87.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.490 - 1.480
R-factor	0.17
Rwork	0.168
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r70
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	1.530
High resolution limit [Å]	1.480	1.480
Number of reflections	40774
<I/σ(I)>	15.8	1.8
Completeness [%]	99.7	98.61
Redundancy	4.7	3.8
CC(1/2)	0.999	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	100 mM bicine, pH 8.5, 25% PEG6000