5T9B
Crystal structure of B. subtilis 168 GlpQ in complex with glycerol-3-phosphate (5 minute soak)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.463, 60.225, 88.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.830 - 1.620 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r70 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.830 | 1.680 |
High resolution limit [Å] | 1.620 | 1.620 |
Number of reflections | 34957 | |
<I/σ(I)> | 19.4 | 1.6 |
Completeness [%] | 99.9 | 99.91 |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100 mM bicine, pH 8.5, 25% PEG6000 |