5T96
Crystal structure of the infectious salmon anemia virus (ISAV) HE viral receptor complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-28 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 |
Unit cell lengths | 82.480, 87.568, 95.534 |
Unit cell angles | 97.14, 113.50, 114.68 |
Refinement procedure
Resolution | 48.396 - 2.000 |
R-factor | 0.1391 |
Rwork | 0.139 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t9y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.400 | 48.400 | 2.030 |
High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
Rmerge | 0.057 | 0.027 | 0.397 |
Rmeas | 0.067 | 0.032 | 0.461 |
Rpim | 0.034 | 0.016 | 0.234 |
Total number of observations | 528952 | 3160 | 26031 |
Number of reflections | 138068 | ||
<I/σ(I)> | 14.9 | 38.1 | 3.5 |
Completeness [%] | 97.9 | 98.5 | 96.6 |
Redundancy | 3.8 | 3.7 | 3.9 |
CC(1/2) | 0.999 | 0.999 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | [0.2 M] magnesium formate, 20% PEG 3350, w/ a [0.8 M] sodium phosphate, [0.8 M] potassium phosphate, [0.1 M] HEPES pH 7.5 solution as a 1:6 ratio drop additive |