5T95
Prephenate Dehydrogenase M219T, N222D mutant from Soybean
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.294, 54.603, 68.092 |
| Unit cell angles | 106.95, 99.32, 103.70 |
Refinement procedure
| Resolution | 33.989 - 1.689 |
| R-factor | 0.1557 |
| Rwork | 0.154 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5T8X |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.980 | 1.715 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 59535 | |
| <I/σ(I)> | 12.31 | 1.7 |
| Completeness [%] | 88.9 | 87.2 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16% (w/v) PEG 8000, 40 mM potassium phosphate dibasic, and 20% (v/v) glycerol |
