5T8U
Crystal structure of P. falciparum LipL1 in complex lipoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127085 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 120.240, 120.240, 134.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.574 - 2.324 |
| R-factor | 0.2742 |
| Rwork | 0.273 |
| R-free | 0.30170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a7a |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2558: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.574 |
| High resolution limit [Å] | 2.324 |
| Rmerge | 0.139 |
| Number of reflections | 48667 |
| <I/σ(I)> | 9.9 |
| Completeness [%] | 96.0 |
| Redundancy | 5.9 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.5M Ammonium Sulfate, 20% Ethylene Glycol |
