5T8L
Crystal structure of Snf7 under 350 MPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.033, 47.467, 45.664 |
Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
Resolution | 32.690 - 2.200 |
R-factor | 0.1975 |
Rwork | 0.191 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fd9 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.817 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.365 | |
Number of reflections | 6489 | |
<I/σ(I)> | 15 | |
Completeness [%] | 99.1 | 91.9 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 6% PEG20000, 110 mM NaCl, 70 mM Mes pH5.5 |