5T85
Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidylglycerol (06:0 PG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-04-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.006, 67.287, 194.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.669 - 2.373 |
R-factor | 0.1979 |
Rwork | 0.195 |
R-free | 0.25040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g6f 3lf6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.107 |
Data reduction software | HKL-2000 (2.3.7) |
Data scaling software | HKL-2000 (2.3.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.669 | 2.400 |
High resolution limit [Å] | 2.373 | 2.373 |
Rmerge | 0.702 | |
Number of reflections | 28788 | |
<I/σ(I)> | 8.5 | 1.8 |
Completeness [%] | 95.7 | 98.1 |
Redundancy | 3.5 | 3.7 |
CC(1/2) | 0.915 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293.15 | 50% PEG 200, 0.1 M Hepes pH 7.0 |