5T7N
X-ray crystal structure of AA13 LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.670, 59.000, 72.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.10804 |
| Rwork | 0.106 |
| R-free | 0.17241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4opb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.075 | 0.456 |
| Number of reflections | 26328 | |
| <I/σ(I)> | 3.14 | |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 6.38 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20%(v/v)PEG3000 0.2M Zn-acetate 0.1M Malate/MES/TRIS pH5.0 |
