5T76
A fragment of a human tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.645, 38.449, 62.471 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.215 |
| R-free | 0.25760 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | |
| Number of reflections | 13269 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.1 M Tris, pH 8.5, 25% w/v PEG3350 |
