Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.757, 72.175, 66.142 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 25.550 - 2.050 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.24040 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ku2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.15) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.180 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 27510 |
| <I/σ(I)> | 0.05 |
| Completeness [%] | 98.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 3350, di Sodium-Tart |
