5T5X

High resolution structure of mouse Cryptochrome 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	43.630, 51.035, 54.175
Unit cell angles	71.62, 84.12, 88.89

Refinement procedure

Resolution	48.428 - 1.840
R-factor	0.1709
Rwork	0.168
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k0r
RMSD bond length	0.013
RMSD bond angle	1.347
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.150	1.860
High resolution limit [Å]	1.800	1.790
Rmerge	0.011
Number of reflections	38528
<I/σ(I)>	45.4
Completeness [%]	93.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M MES pH 6.8, 10 mM EDTA, 15% v/v PEG3350