5T5K
Structure of histone-based chromatin in Archaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.454, 99.454, 171.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.760 - 4.000 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a7w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.145 | 0.517 |
| Number of reflections | 8122 | |
| <I/σ(I)> | 19.9 | 5.7 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 100 mM MgAc, 50 mM Na Cacodylate pH 6.5, 10% PEG 400 under silicon oil |
