5T5D

Crystal Structure of the PTS IIB protein associated with the fucose utilization operon from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9761
Spacegroup name	P 65 2 2
Unit cell lengths	42.503, 42.503, 292.922
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.809 - 2.500
R-factor	0.2277
Rwork	0.224
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.958
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.000
High resolution limit [Å]	2.500
Number of reflections	67795
<I/σ(I)>	14.9
Completeness [%]	99.2
Redundancy	10.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	30% PEG400, 0.2 M Magnesium chloride