5T5B

CRYSTAL STRUCTURE OF THE COMPLEX OF 10E8 FAB LIGHT CHAIN MUTANT5 AND T117V2 HIV-1 MPER SCAFFOLD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	40.931, 159.047, 100.606
Unit cell angles	90.00, 95.17, 90.00

Refinement procedure

Resolution	37.880 - 2.070
R-factor	0.18
Rwork	0.178
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4G6F AND 3LF6
RMSD bond length	0.008
RMSD bond angle	0.879
Data reduction software	HKL-2000 (Version 2.3.10)
Data scaling software	HKL-2000 (Version 2.3.10)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.883	2.110
High resolution limit [Å]	2.067	2.070
Rmerge		0.456
Number of reflections	73937
<I/σ(I)>	9.2	2.1
Completeness [%]	94.8	91.4
Redundancy	2.1	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295.15	0.1 M Hepes pH 7.0, 30% PEG 6000;