5T5A
Crystal Structure of the Twister Sister (TS) Ribozyme at 2.0 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2016-05-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.91772 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 39.304, 39.304, 228.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.160 - 2.000 |
R-factor | 0.1721 |
Rwork | 0.170 |
R-free | 0.21710 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.987 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX ((1.10.1_2155: ???)) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.160 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.376 |
Number of reflections | 268378 | |
<I/σ(I)> | 28.95 | |
Completeness [%] | 97.0 | |
Redundancy | 21.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 289 | magnesium acetate sodium cacodylate MPD |