5T4W
Crystal structure of a DNA sequence d (CGTGAATTCACG) with DAPI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2016-04-20 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.503, 41.090, 65.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.760 - 2.300 |
| R-factor | 0.2203 |
| Rwork | 0.216 |
| R-free | 0.25839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ju4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.559 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.760 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.080 |
| Number of reflections | 2915 |
| <I/σ(I)> | 2.88 |
| Completeness [%] | 90.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 60mM NaCaCo, 17mM MgCl2, 2mM Spermine, 50%MPD, 1.5 mM DAPI |
