5T4L

PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-09
Detector	MAR CCD 130 mm
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	84.913, 154.916, 79.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.990 - 1.530
R-factor	0.15
Rwork	0.149
R-free	0.16600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amq
RMSD bond length	0.007
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.000
High resolution limit [Å]	1.530
Number of reflections	77078
<I/σ(I)>	34
Completeness [%]	98.2
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25 mM Potassium phosphate 43% Ammonium sulfate