5T4J

PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	92.364, 128.894, 65.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.183 - 2.231
R-factor	0.1744
Rwork	0.172
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mgr
RMSD bond length	0.005
RMSD bond angle	0.862
Data reduction software	HKL-2000
Refinement software	PHENIX (dev_1563)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.200
High resolution limit [Å]	2.230
Number of reflections	104767
<I/σ(I)>	29.3
Completeness [%]	99.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M Tris (pH:8.5) and 20% ethanol