5T40

A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-11
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	73.368, 83.732, 74.999
Unit cell angles	90.00, 113.54, 90.00

Refinement procedure

Resolution	34.703 - 1.810
R-factor	0.164
Rwork	0.162
R-free	0.18670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ism
RMSD bond length	0.004
RMSD bond angle	0.694
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.840
High resolution limit [Å]	1.810	4.910	1.810
Rmerge	0.052	0.018	0.814
Rmeas	0.062
Rpim	0.032
Total number of observations	266281
Number of reflections	74300
<I/σ(I)>	11.3
Completeness [%]	98.1	98.6	97.8
Redundancy	3.6	3.7	3.4
CC(1/2)		0.999	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	2.0M (NH4)2SO4, 0.1M Tris-HCl pH 8.5, 0.1M MgCl2