5T3V
A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1.89 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.340, 83.436, 74.771 |
| Unit cell angles | 90.00, 113.42, 90.00 |
Refinement procedure
| Resolution | 49.708 - 2.600 |
| R-factor | 0.1798 |
| Rwork | 0.176 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ism |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.708 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.060 |
| Number of reflections | 24211 |
| <I/σ(I)> | 21.7 |
| Completeness [%] | 95.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2M (NH4)2SO4, 0.1M MgCl2, 0.1M Tris-HCl pH 8.5 |
