5T3U
Crystal Structure of the PTS IIA protein associated with the fucose utilization operon from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-01-20 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 100.202, 100.202, 192.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.150 |
R-factor | 0.20615 |
Rwork | 0.204 |
R-free | 0.25277 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.391 |
Data reduction software | CrystalClear |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.060 |
Number of reflections | 197784 |
<I/σ(I)> | 15.9 |
Completeness [%] | 95.2 |
Redundancy | 7.67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG3350, 0.2M ammonium sulfate, 0.1M citrate pH 5.8 |