5T3K
Fluorescence detection of RNA-ligand binding and crystal structure determination of ribosomal decoding site RNA using a heavy atom containing fluorescent ribonucleoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-02-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.553, 86.956, 32.564 |
Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
Resolution | 26.020 - 2.142 |
R-factor | 0.2028 |
Rwork | 0.197 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (dev_2313) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.020 | 2.220 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 7483 | |
<I/σ(I)> | 11.32 | 4.25 |
Completeness [%] | 77.6 | 46.7 |
Redundancy | 2.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.2mM RNA, 2.40-2.55M ammonium sulfate, 10mM magnesium acetate, 40mM2-(N-morpholino)ethanesulfonic acid (MES) buffer, pH 5.6 |