5T3I
cyan fluorescence protein soaked with selenourea for 5 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-27 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.116, 61.520, 69.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.760 - 1.600 |
R-factor | 0.136 |
Rwork | 0.134 |
R-free | 0.17700 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.674 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 | 1.280 |
Number of reflections | 30369 | |
<I/σ(I)> | 24.7 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 16% (w/v) PEG 3350, 50 mM citric acid, and 50 mM bis-tris propane buffer pH 5.0. |