5T3A

Maedi-Visna virus (MVV) integrase CCD-CTD (residues 60-275)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	C 2 2 21
Unit cell lengths	78.050, 124.830, 62.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.415 - 2.501
R-factor	0.1823
Rwork	0.179
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hpg 5llj
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.420	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.129	1.540
Number of reflections	10859
<I/σ(I)>	13	1.7
Completeness [%]	99.7	98.4
Redundancy	9.4	9.6
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1M Mes pH6.0, 15-100mM calcium acetate and 15-21% PEG 400