5T38
Crystal Structure of the N-terminal domain of EvdMO1 with SAH bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.427, 41.272, 56.465 |
Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
Resolution | 32.002 - 1.150 |
R-factor | 0.1536 |
Rwork | 0.153 |
R-free | 0.16820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mgg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.170 |
High resolution limit [Å] | 1.150 | 3.120 | 1.150 |
Rmerge | 0.052 | 0.029 | 0.347 |
Number of reflections | 79855 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 96.3 | 95.7 | 70 |
Redundancy | 3.5 | 3.5 | 2.3 |
CC(1/2) | 0.998 | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40% Tacsimate, pH 7.4 |