5T2R

Unliganded Human HVEM at 2.1A in P 21 21 21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	47.210, 62.420, 103.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.27712
Rwork	0.275
R-free	0.32144
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aw2
RMSD bond length	0.018
RMSD bond angle	2.003
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.111
Number of reflections	17307
<I/σ(I)>	16	2.7
Completeness [%]	98.8	94.2
Redundancy	7.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296.15	0.15 M HEPES 7.5, 0.15 M NaCl, 0.05 M AS, 30% (w/v) PEG 8K, 5% (v/v) Glycerol