5T2I
mPI3Kd IN COMPLEX WITH 7k
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.976 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.887, 142.812, 220.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.320 - 2.300 |
R-factor | 0.237 |
Rwork | 0.234 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.320 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.076 | 0.770 |
Number of reflections | 44552 | |
<I/σ(I)> | 14.7 | 3.1 |
Completeness [%] | 98.7 | 98 |
Redundancy | 6.6 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50% ethylene glycol/PEG 8000, 0.1 M carboxylic acids mix, 0.1 M Buffer system 2 pH 7.5 |