5T23
PI3Kg IN COMPLEX WITH 5d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.150, 68.210, 106.810 |
Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
Resolution | 41.060 - 2.780 |
R-factor | 0.25902 |
Rwork | 0.255 |
R-free | 0.33923 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.066 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.770 | 2.850 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.500 | |
Number of reflections | 26068 | |
<I/σ(I)> | 19.1 | 2.1 |
Completeness [%] | 98.8 | 99.1 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Hepes pH 7.7-8.2, 18-19% PEG3350, 175mM (NH4)2SO4, 10mM DTT |