5T1Y
MLA10 coiled-coil fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.720, 87.140, 92.247 |
| Unit cell angles | 89.93, 90.00, 89.98 |
Refinement procedure
| Resolution | 40.785 - 2.050 |
| R-factor | 0.2543 |
| Rwork | 0.252 |
| R-free | 0.27880 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.348 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (dev_2376) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.047 | 0.037 |
| Number of reflections | 58095 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 96.5 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Pact Premier, condition B4 (MIB buffer pH 7.0, 25% PEG 1500) |
