5T1X
Crystal Structure of Native Tarin Lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-23 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.700, 82.710, 122.700 |
Unit cell angles | 90.00, 91.76, 90.00 |
Refinement procedure
Resolution | 17.140 - 1.700 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dlp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.040 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.067 | 0.381 |
Number of reflections | 104443 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.3 | 98.1 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 25-35% Peg 3350, 0.2M Lithium Sulfate, 0.1M Hepes |