5T1P
Crystal structure of the putative periplasmic solute-binding protein from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 82.190, 89.276, 100.394 |
Unit cell angles | 68.88, 82.52, 70.66 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.17815 |
Rwork | 0.176 |
R-free | 0.22393 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.730 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.690 |
Number of reflections | 167225 | |
<I/σ(I)> | 21.8 | 3.7 |
Completeness [%] | 98.1 | 97 |
Redundancy | 11.8 | 11.5 |
CC(1/2) | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% P3350, 0.1M B-Tris pH 6.5, MgSulphate 1mM |