5T1E
Crystal structure of Phaeospaeria nodrum fructosyl peptide oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.014, 93.476, 47.877 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.830 |
| R-factor | 0.1446 |
| Rwork | 0.142 |
| R-free | 0.18942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rsl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.136 | 0.461 |
| Number of reflections | 33853 | |
| <I/σ(I)> | 8.9 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 18-22 % (w/v) PEG 3350, 8 % (w/v) Tacsimate |
