5T1D
Crystal structure of EBV gHgL/gp42/E1D1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.510, 166.000, 272.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.110 - 3.100 |
R-factor | 0.2143 |
Rwork | 0.212 |
R-free | 0.26500 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.634 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.110 | 3.005 |
High resolution limit [Å] | 2.901 | 2.901 |
Rmerge | 0.123 | 1.590 |
Number of reflections | 53195 | |
<I/σ(I)> | 12.21 | 1.6 |
Completeness [%] | 99.8 | 99.05 |
Redundancy | 5.9 | 5.9 |
CC(1/2) | 0.997 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris pH 8.0, 0.18 M Potassium Citrate, 12 % PEG 6000 |