5T12
N-terminal domain of Enzyme 1 - Nitrogen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 65 |
Unit cell lengths | 64.770, 64.770, 123.692 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.221 - 2.299 |
R-factor | 0.2152 |
Rwork | 0.213 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zym |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.221 | 2.380 |
High resolution limit [Å] | 2.299 | 2.299 |
Rmerge | 0.112 | 0.509 |
Number of reflections | 11880 | |
<I/σ(I)> | 12.8 | 1.48 |
Completeness [%] | 96.2 | 90.3 |
Redundancy | 4.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 1.0M Potassium/Sodium tartrate 0.1M imidazole 8.0 0.2M NaCl 1.0M sodium iodide |