5T0Y
2A10 Antibody FAB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.032900 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 231.684, 231.684, 81.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.733 - 3.011 |
| R-factor | 0.2259 |
| Rwork | 0.225 |
| R-free | 0.24670 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.733 | 3.120 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Number of reflections | 44655 | |
| <I/σ(I)> | 10.6 | 1.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 14.6 | 14.6 |
| CC(1/2) | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 2M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5 |
