5T0V
Architecture of the Yeast Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Sub-Complex Formed by the Iron Donor, Yfh1, and the Scaffold, Isu1
EM specimen
| Staining | Yes |
| Staining material | uranyl acetate |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 17.5 |
| Number of Particle | 4153 |
| Software | EMAN2,NAMD,Coot,DigitalMicrograph,UCSF Chimera,Situs |
| Symmetry | O |
| CTF correction | PHASE FLIPPING ONLY |
