5T0S
Synaptotagmin 1 C2B domain, cadmium-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-07 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.992, 41.948, 80.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.540 - 1.420 |
| R-factor | 0.1624 |
| Rwork | 0.159 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uow |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.950 | 1.490 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.544 | |
| Number of reflections | 26944 | |
| <I/σ(I)> | 18.7 | 2.6 |
| Completeness [%] | 99.0 | 94.3 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 13.4% PEG 400, 10.05% PEG 1000, 0.15 M potassium phosphate dibasic/sodium phosphate dibasic pH 6.5 |
