5T0R
Synaptotagmin 1 C2A domain, cadmium-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-04 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.064, 63.276, 76.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.070 - 1.950 |
| R-factor | 0.1906 |
| Rwork | 0.186 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f00 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.070 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.373 | |
| Number of reflections | 10075 | |
| <I/σ(I)> | 13 | 3.2 |
| Completeness [%] | 96.9 | 85 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.01 M tri-sodium citrate, 16% PEG 6000 |
