5T0O
Crystal Structure of a membrane protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 300.710, 147.538, 120.027 |
Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
Resolution | 84.930 - 3.150 |
R-factor | 0.2139 |
Rwork | 0.211 |
R-free | 0.26780 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.260 |
High resolution limit [Å] | 3.150 | 6.790 | 3.150 |
Rmerge | 0.103 | 0.024 | 0.483 |
Total number of observations | 251867 | ||
Number of reflections | 158913 | ||
<I/σ(I)> | 6.7 | ||
Completeness [%] | 90.7 | 89.5 | 90.7 |
Redundancy | 1.6 | 1.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 4% PEG8000, 0.1M MgSo4, 0.1M MES (6.5) |