5T08
Crystal structure of H6 hemagglutinin G225D mutant from Taiwan (2013) H6N1 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.065, 98.364, 133.239 |
Unit cell angles | 90.00, 126.48, 90.00 |
Refinement procedure
Resolution | 49.339 - 2.189 |
R-factor | 0.2172 |
Rwork | 0.215 |
R-free | 0.25010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xkd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.635 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.189 | 2.189 |
Number of reflections | 96720 | |
<I/σ(I)> | 19.1 | |
Completeness [%] | 98.5 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 10mM NiCl2, 0.1M Tris pH 8.5, 20% (w/v) MPEG 2000 and 20% glycerol |