5SZU
Novel Structural Insights into GDP-Mediated Regulation of Acyl-CoA Thioesterases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 153.198, 153.198, 153.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.445 - 2.800 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ien |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.567 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.123 | 0.574 |
| Number of reflections | 28972 | |
| <I/σ(I)> | 6.45 | 1.96 |
| Completeness [%] | 97.4 | 99.66 |
| Redundancy | 3.5 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 100 mM Tris pH 8.5 and 2 M ammonium phosphate |
