5SZR
Protocadherin Gamma B2 extracellular cadherin domains 3-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97919 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.755, 104.755, 352.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.949 - 2.300 |
R-factor | 0.2513 |
Rwork | 0.250 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dzv |
RMSD bond length | 0.004 |
RMSD bond angle | 0.650 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.119 | 1.886 |
Number of reflections | 87920 | |
<I/σ(I)> | 6 | 0.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.5 | 5.6 |
CC(1/2) | 0.998 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 295 | 11.5% (w/v) PEG8000, 23% (v/v) ethylene glycol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1 M Morpheus Buffer System 1 (Mes/Imidazole buffer; Molecular Dimensions) pH 6.5 |